Journal: Molecular Vision

Article Title: Reduction in ocular complement factor B protein in mice and monkeys by systemic administration of factor B antisense oligonucleotide

doi:

Figure Lengend Snippet: Reduction in ocular factor B protein following trans-cardial perfusion of wild-type mice. C57BL/6J mice were subjected to trans-cardial perfusion with PBS. Unperfused mice served as control (n = 3 per group). A : The factor B protein levels were measured in the ocular extracts with western blot analysis. Total protein extracts were obtained from one whole eye. Different amounts of recombinant human factor B protein (rhFB) were loaded on the gel to estimate the ocular factor B levels. Immunoblotting for tubulin was used to ensure equal protein loading on the gel. B : Quantification of panel A. C : Analysis of liver factor B protein levels with western blot. Immunoblotting for tubulin was used to ensure equal protein loading on the gel. D : Quantification of panel C.

Article Snippet: In the perfusion experiments, 9-week-old, wild-type C57BL/6J mice were subjected to trans-cardial perfusion with Dulbecco’s PBS (137.9 mM NaCl, 2.7 mM KCl, 8 mM Na 2 HPO 4 , 1.5 mM KH 2 PO 4 , pH7.3; DPBS, no calcium, no magnesium; Invitrogen, Thermo Fisher Scientific, Cat.: 14190144) to remove the blood from the tissues.

Techniques: Control, Western Blot, Recombinant